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Transfection is a crucial laboratory technique used in molecular biology to introduce nucleic acids (DNA or RNA) into cells. It plays a pivotal role in gene expression studies, genetic engineering, and therapeutic research. The efficiency of transfection largely depends on the choice of transfection reagents and equipment, which vary based on cell type, nucleic acid size, and the desired application. This article explores the key types of transfection reagents and equipment used in modern laboratories.
Transfection can be classified into two broad categories:
Stable Transfection: Involves the integration of foreign genetic material into the host genome, ensuring long-term gene expression.
Transient Transfection: Allows temporary gene expression without genome integration, typically lasting a few days.
